Making and Using Antibodies: A Practical Handbook

Antibodies are an indispensable tool in the study of biology and medicine. Making and Using Antibodies: A Practical Handbook presents techniques in a single, comprehensive source for the production and use of antibodies. It enables researchers to immediately access lab-tested, proven protocols. Written and edited by an elite team of scientists, who have developed and refined many of the methods, this book covers-Commercially available adjuvants designed for the production of antisera in the research settingMethods for the production, purification, and characterization of antibodies Practical guidance to researchers needing to modify antibodiesBasic techniques including enzyme-linked immunosorbent assay (ELISA), Western blotting, immunohistochemistry, and flow cytometryMethods for applying immunolabeling to the realm of electron microscopy (EM)New antibody sources and new ways to use antobodies Making and Using Antibodies: A Practical Handbook also offers invaluable insight into future directions, challenges, and opportunities both in research and industrial applications.